Manipulating the Mouse Embryo: A Laboratory Manual, Fourth Edition (1936113015)
This 4th edition of the 'Mouse Manual'-
Manipulating the Mouse Embryo-appears 28 years after the first edition and once again is the definitive reference source on mouse development, transgenesis techniques, and molecular biology. While many of the techniques described in earlier editions of this manual have been relegated to core facilities, advances in DNA sequencing techniques and genome analysis have opened new avenues of research and developments. New approaches, such as the derivation of induced pluripotent stem cells and new targeted gene manipulation techniques that enable direct injection of RNA/DNA constructs into zygotes to achieve gene targeting, require new explanations and protocols. Authors Richard Behringer, Marina Gertsenstein, Kristina Nagy, and Andras Nagy-pre-emininent leaders in their fields-have taken these developments into account in updating and rewriting this fourth edition to include new information and protocols on:
- generation of induced pluripotent stem cells
- RNA microinjections
- lentiviral microinjections and infection
- assisted reproduction techniques for sperm and embryo cryopreservation
- isolation, generation, and transplantation of spermatogonial stem cell lines
- in utero electroporation of gene constructs into postimplantation embryos
- vibratome sectioning of live and fixed tissues for imaging thick tissue sections
- whole-mount fluorescent staining methods for three-dimensional visualization
In addition, the wealth of essential information on mouse laboratory strains, mouse housing and breeding, surgical procedures, assisted reproduction, handling of embryos, and micromanipulation setups has been entirely updated. The first edition of
Manipulating the Mouse Embryo appeared in 1986 as an outgrowth of Cold Spring Harbor Laboratory courses on the molecular embryology of the mouse held in the early 1980s, and authors of the first two editions included Brigid Hogan, Rosa Beddington, Frank Costantini, and Liz Lacy. The field's technological sophistication has grown exponentially since then, but the manual remains the essential practical and theoretical guide for all students, lab technicians, and investigators who work with mice.
I have the third edition. Why should I buy the fourth edition?The new technologies regarding induced pluripotent stem cells and RNA injection and CRISPR technology are changing the field and opening up new avenues of investigation. The protocols for these as well as other up-to-the minute techniques in the fourth edition are by the leading proponents in the field and offer clear step-by-step guidance with copious illustrations.
To whom is this edition addressed-the neophyte or the experienced investigator?Both groups can benefit from this manual. For the neophyte, the summary of mouse development and the how-to's of setting up a working laboratory are an invaluable introduction
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